Additional analysis will allow a better understanding of the expression profile and discovering the key genes in this life cycle. ![]() The UTR size of Leishmania and the lack of CATG sites in some transcripts were decisive for the generation of tags in these regions. Only 5.2% (84/1,645) of the tags were not aligned to any position in the L. BLAST research of the 1,645 most abundant tags showed that 12.8% of them identified the coding sequences of genes, while 82% (1,349/1,645) identified one or more genomic sequences that did not correspond with open reading frames. A tag library was created using the LongSAGE method and consisted of 14,208 tags of 17 bases. This study describes the application of the LongSAGE methodology to study the gene expression profile in promastigotes of Leishmania infantum chagasi.
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